Circular Dichroism (CD) spectra are highly sensitive to distortions within the chiral centers of biopolymers and help analyze the secondary structure of biopolymers, especially proteins/peptides and nucleic acids (Rogers et al., 2019). CD spectroscopy is the most common approach for studying the overall conformation of biopolymers, such as proteins and peptides.
The CD is a spectroscopic method based on the interaction of chiral centers in the polymer with circularly polarized light, which is very sensitive to levels of regular order in the polymer backbone. CD offers a convenient method for identifying changes in the structure of proteins and peptides.
- Consultation on sample conditions and spectral acquisition parameters
- Sample preparation, including sample concentration and exchange into CD-compatible buffer.
- Recording the CD spectra with optimal parameters and temperatures (if temperature-dependent studies are needed).
- Collection and plotting of data
- Computational analysis of protein secondary structure
- Written report of results
